• Users Online: 125
  • Home
  • Print this page
  • Email this page
Home About us ASMR Conference Editorial board Search Ahead of print Current issue Archives Submit article Instructions Subscribe Contacts Login 
Year : 2012  |  Volume : 7  |  Issue : 2  |  Page : 48-56

Image analysis assessment of fibrosis from liver biopsy of chronic hepatitis patients

1 Pathology Department, Faculty of Medicine, Cairo University, Cairo, Egypt
2 Pathology Department, National Research Centre, Dokki, Cairo, Egypt

Correspondence Address:
Abdel Razik H Farrag
PhD, Pathology Department, National Research Centre, Dokki, 12622 Cairo
Login to access the Email id

Source of Support: None, Conflict of Interest: None

Rights and PermissionsRights and Permissions

Background/aim Liver fibrosis results from chronic inflammation of the hepatic parenchyma. Progressive accumulation of fibrous tissue eventually leads to cirrhosis and its complications. The severity of liver fibrosis defines the stage of chronic hepatitis and carries with it important clinical implications. Histological scoring systems such as Ishak provide descriptive evaluation of the liver tissue mainly in terms of architectural changes without measuring the amount of fibrosis. This study aimed to assess the theoretical advantage of image analysis morphometry for providing truly quantitative data with respect to the amount of fibrous tissue. Materials and methods A Lieca Qwin 500 image analyzer with a damaged area morphometry software was used applying the interactive method to measure the fibrous tissue area on the basis of different colors of hepatocytes and fibers following staining with Masson's trichrome stain. Forty-three patients (38 men and five women) were recruited into the study with a mean age of 45.5 years (range 15-58 years). Of them, 40 had chronic viral hepatitis and three had chronic nonviral hepatitis. Results Morphometric measurements of fibrosis obtained with the image analyzer were highly correlated with results obtained using the Ishak method. The correlation was found to be statistically significant using the w2-test (Po0.0001). Conclusion Quantitative image analysis for estimation of the percentage area of liver fibrosis is a simple and accurate method for evaluating fibrous tissue in patients with chronic hepatitis, aiding therapeutic approaches.

Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)

 Article Access Statistics
    PDF Downloaded59    
    Comments [Add]    

Recommend this journal